References: Lecithin
J Biochem (Tokyo). 1992 Mar;111(3):413-8.
Interaction of rat lecithin-cholesterol acyltransferase with rat apolipoprotein A-I and with lecithin-cholesterol vesicles.
Furukawa Y, Urano T, Hida Y, Itoh H, Takahashi C, Kimura S.
Department of Food Chemistry, Faculty of Agriculture, Tohoku University, Miyagi.
The interaction of rat plasma lecithin-cholesterol acyltransferase with lecithin-cholesterol vesicles and with rat apo-A-I was studied in comparison with that of human plasma lecithin-cholesterol acyltransferase to clarify the reaction mechanism of rat plasma lecithin-cholesterol acyltransferase. The interaction of both human and rat lecithin-cholesterol acyltransferase with lecithin-cholesterol vesicles was investigated by gel permeation chromatography on Superose 12. Both enzymes had almost the same affinity to the vesicles. The affinity of rat enzyme to rat apo-A-I was stronger than that of human enzyme to human apo-A-I when estimated on the apo-A-I-Sepharose 4B column. When human apo-A-I was added to the human enzyme/vesicle mixture which contained the enzyme-vesicle complex, the enzyme was effectively dissociated from the complex. But when rat apo-A-I was added to the rat enzyme/vesicle mixture, apo-A-I-enzyme-vesicle complex was still recognized by its elution pattern on gel permeation chromatography. This suggests that the mixture of rat enzyme, rat apo-A-I, and vesicles, which are the major components in the rat lecithin-cholesterol acyltransferase reaction, forms a stronger complex than do the components of the human reaction.
Laxative online source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=1587806&dopt=Abstract lecithin
Acta Genet Med Gemellol (Roma). 1994;43(3-4):185-92.
Evaluation of amniotic fluid elastolytic activity: can it be a method of fetal lung maturity assessment? A comparison with Gluck's L/S test.
Bazowska G, Jendryczko A, Dudkiewicz J.
2nd Department of Obstetrics and Gynecology, Silesian School of Medicine.
Amniotic fluid elastolytic activity was assessed in a group of 120 women who delivered preterm infants and in 35 women who delivered at term. Amniotic fluid elastolytic activity decreases as pregnancy progresses. The lecithin-to-sphingomyelin (L/S) ratio in women's amniotic fluid was determined by the method developed by Gluck and associates [6] and elastolytic activity by that developed by Mehdi and associates. A significant negative correlation was found between the amniotic fluid L/S ratio and amniotic fluid elastolytic activity (r = -0.932; p < 0.001). The border value of elastolytic activity that indicates lung maturity (L/S ratio equal to or greater than 2) is 2.01 +/- 0.05 mmol/min ml. In the amniotic elastolytic activity test, it is the value that differentiates mature from immature lungs. The amniotic fluid elastolytic activity test is characterized by high sensitivity (91.43%) and specificity (91.67%), high positive prognostic value (76.19%) and low negative prognostic value (2.65%). The test parameters do not therefore differ greatly from those of the Gluck test. Moreover, the amniotic fluid elastolytic activity test is cheaper and takes less time to perform.
Laxative online source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8588493&dopt=Abstract lecithin
J Intern Med. 1992 Apr;231(4):413-9. ["OMIM","window.top.location='Laxative online source: www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?db=PubMed&cmd=Display&dopt=pubmed_omim&from_uid=1588268'","",""],
Familial high-density-lipoprotein deficiency causing corneal opacities (fish eye disease) in a family of Dutch descent.
Kastelein JJ, Pritchard PH, Erkelens DW, Kuivenhoven JA, Albers JJ, Frohlich JJ.
Centre for Haemostasis, Thrombosis and Atherosclerosis, University of Amsterdam, The Netherlands.
Fish eye disease (FED) is an extremely rare familial disorder characterized by severe HDL deficiency and extensive corneal opacities. This disorder appears to be a variant of familial lecithin: cholesterol acyltransferase (LCAT) deficiency in which the enzyme remains partly active yet the ability of the enzyme to esterify cholesterol in high-density lipoprotein (HDL) has been lost. The rarity of this disorder has limited advances in our understanding of the pathophysiology of the HDL deficiency. However, we here describe the clinical and biochemical presentation of a family with FED who are of Dutch descent. The proposition presented with HDL deficiency and corneal opacity. Subsequently, they were diagnosed as having FED by the absence of LCAT activity against a small proteoliposome substrate despite the presence of half-normal LCAT mass and a near-normal ratio of unesterified to total cholesterol in plasma. Heterozygotes presented with half-normal LCAT activity, but not with decreased HDL. With the identification of this three-generation family, renewed investigation of this intriguing disorder of HDL is now possible.
Laxative online source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=1588268&dopt=Abstract lecithin
Buy Rx Online || Hair Million herbal formula for hair loss and hair growth || Hair Million, excellent herbal formula, wards off hair loss and promotes hair growth || Buy Tramadol || Lecithin product online guide ||