References: Lecithin
Biochemistry. 1993 May 25;32(20):5425-30.
Acyl group transfer from the sn-1 position of phospholipids in the biosynthesis of n-dodecyl palmitate.
Furuyoshi S, Shi YQ, Rando RR.
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115.
The wax ester n-dodecyl palmitate is shown to be synthesized by retinal pigment epithelial membranes. The biosynthesis of this ester is phospholipid dependent and occurs via the transfer of a palmitoyl group from the sn-1 position of lecithin to n-dodecanol. When retinal pigment epithelial membranes are used as the source of enzyme, the apparent Michaelis constant for n-dodecanol in this process is 65.8 microM, and the maximal velocity for n-dodecyl palmitate synthesis is 16.2 nmol/(h.mg of protein). The enzymatic activity is membrane associated and shows a maximum velocity between pH8 and pH9. This transesterification process appears to be similar to the lecithin retinol acyl transferase reaction and is a further example of acyl group transfer reactions from the sn-1 position of phospholipids.
Laxative online source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8499446&dopt=Abstract lecithin
Afr J Med Med Sci. 2001 Sep;30(3):157-60.
Quantitative effects of interacting variables on vitamin K1, phylloquinone, entrapment in liposomes.
Onaga IC, Oyenuga KO.
Department of Pharmaceutics and Industrial Pharmacy, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Vitamin K1 is poorly absorbed by the oral route in certain diseased conditions. Entrapment of this vitamin in liposomes is expected to improve oral absorption. In this study, vitamin K1 has been encapsulated into multilamellar vesicles (MLVs) composed of either egg phosphatidylcholine (egg PC, EPC) or dipalmitoyllecithin (DPPC) by the classical film method involving hydration of lipids by either hand shaking or vigorous vortex mixing. Amounts of vitamin K1 ranging from 4.4 to 22.1 microMol were employed to medicate liposome suspensions (26 microMm or 13.6 microM). A 2(3) factorial design was employed to quantitative the effects of the interaction of certain variables viz: nature of lipid (L), method of agitation (M) and initial drug concentration (C), on encapsulation capacity (EC) of liposomes. The results of this study suggest, in particular, that the concentration of the drug (C) and the nature of the lipid-drug interactions (L-C) are the most influential variables. This probably infers that any pre-formulation studies with this drug delivery system should start with a consideration of L-C interaction. A negative correlation was found between initial drug concentration and encapsulation capacity of liposomes.
Laxative online source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=14510120&dopt=Abstract lecithin
Biotechnol Lett. 2003 Sep;25(17):1425-9.
Interaction of sodium nitroprusside/liposome system with bovine serum albumin: a short-cut determination.
Taira MC, Stern AL, Escobar ME, Adragna N, Alonso-Romanowski S.
Laboratorio de Biomembranas, Departmento de Ciencia y Tecnologia, Universidad Nacional de Quilmes, BXD1876B Bernal, Argentina.
A rapid determination of protein-liposome binding was developed to predict the circulation time of the system within an animal, which is a function of the amount and type of protein bound. The binding pattern of albumin to liposomes, with and without sodium nitroprusside (SNP), was analyzed by SDS-PAGE. Liposomes were made of egg yolk lecithin, soybean lecithin and dimyristoyl lecithin, and contained SNP. They bound 58%, 26% and 100% bovine serum albumin, respectively, when compared to their corresponding controls lacking SNP. The method applied is simpler and significantly faster than ordinary chemical determinations.
Laxative online source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=14514044&dopt=Abstract lecithin [PubMed - in process]
Lecithin online literature || Lecithin online literature || Lecithin online literature || Lecithin online literature || Lecithin online literature || Lecithin online literature || Lecithin online literature
Buy Rx Online || Hair Million herbal formula for hair loss and hair growth || Hair Million, excellent herbal formula, wards off hair loss and promotes hair growth || Buy Tramadol || Dream Pharmaceuticals Online Pharmacy: Buy Rx Online || Lecithin product online guide ||